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Beclin 1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | Q14457 |
| Other Accession | NP_001300927.1 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human Beclin 1 Polyclonal |
| Target/Specificity | Predicted molecular weight at ~51kDa. Observed a secondary band on some lysates at ~30kDa. |
| Other Names | APG6 Antibody, BCL-2 interacting protein beclin Antibody, Beclin 1 autophagy related Antibody, BECN1 Antibody, BECN1_HUMAN Antibody, GT197 Antibody, VPS30 Antibody |
| Immunogen | Synthetic peptide from the mid-protein of human Beclin 1 |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-600 was sufficient for detection of Beclin1 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm | Golgi apparatus | Trans-Golgi network membrane| Endosome | Endoplasmic reticulum membrane | Mitochondrion | Mitochondrion membrane | Cytoplasmic vesicle | Autophagosome |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Beclin 1 is the mammalian ortholog of the yeast autophagy -related gene Atg6. It regulates autophagy and has an important role in development, tumorigenesis and neurodegeneration (1). Beclin 1 is localized within cytoplasmic structures including the mitochondria, yet overexpression reveals some nuclear staining (2). Researchers have found that schizonphrenia is associated with low levels of Beclin-1 in the hippocampus (3). It may also protect against infection by a neuorvirulent strain of Sindbis virus (4).
References
1. Zhong Y., et al. (2009) Nat Cell Biol. 11(4): 468-476.
2. Liang X.H., et al. (2001) Cancer Res. 61: 3443-3449.
3. Merenlender-Wagner A., et al. (2013) Mol. Psychiatry 20:126-132.
4. Liang X.H., et al. (1998) J Virol. 72: 8586-8596.
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