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GSK-3beta Antibody

     
  •  - GSK-3beta Antibody ASM10424
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
    detail
  • WB - GSK-3beta Antibody ASM10424
    Western blot analysis of Human Cell line lysates showing detection of GSK3 Beta protein using Rabbit Anti-GSK3 Beta Polyclonal Antibody (ASM10424). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-GSK3 Beta Polyclonal Antibody (ASM10424) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
    detail
  •  - GSK-3beta Antibody ASM10424
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (ASM10424) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P49841
Other Accession NP_001139628.1
Host Rabbit
Reactivity Human, Mouse, Rat, Bovine
Clonality Polyclonal
Description Rabbit Anti-Human GSK-3beta Polyclonal
Target/Specificity Detects ~47kDa.
Other Names Glycogen Synthase kinase 3 beta Antibody, GSK 3 beta Antibody, GSK 3B Antibody, GSK-3 beta Antibody, GSK3B Antibody, GSK3B_Human Antibody, GSK3beta isoform Antibody
Immunogen Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cell Membrane
Research Areas
Citations (0)
citation

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Background

Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues.  It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer’s, cancer and bipolar (4, 5).

References

1. Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595.
2. Wang H. (2011) Cytokind. 53(2): 130-140.
3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47.
4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063.
5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.

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Cat# ASM10424
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