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O-GalNAc Antibody

O-GalNAc Antibody, Clone 9B9

     
  •  - O-GalNAc Antibody ASM10331
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9 (ASM10331). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody (ASM10331) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
    detail
  • WB - O-GalNAc Antibody ASM10331
    Western Blot analysis of Glycoconjugates showing detection of 67 kDa GalNAc-BSA using Mouse Anti-GalNAc Monoclonal Antibody, Clone 9B9 (ASM10331). Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-GalNAc Monoclonal Antibody (ASM10331) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Host Mouse
Isotype IgM
Clonality Monoclonal
Description β-O-Linked N-Acetylgalactosamine Antibody
Target/Specificity Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
Other Names GalNAC Antibody, O-GalNAC Antibody, β-O-Linked N-Acetylgalactosamine Antibody, O-Linked N-Acetylgalactosamine Antibody, N-Acetylgalactosamine Antibody, 2-(Acetylamino)-2-deoxy-D-galactose Antibody, 2-Acetamido-2-deoxy-D-galactose Antibody, N-Acetylchondrosamine Antibody, 2-Acetamido-2-deoxy-D-galactopyranose Antibody, N-Acetyl-D-galactosamine Antibody, alpha-N-Acetylgalactosamine Antibody, N-Acetylgalactosamine-modified protein Antibody
Trademark MOLECULAR SIGNATURE®
Clone Names 9B9
Immunogen Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Purification PEG Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-503 was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Cat# ASM10331
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