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Synaptotagmin-12 Antibody
Synaptotagmin 12 Antibody, Clone S277-7
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Application 
| WB, IHC, ICC |
|---|---|
| Primary Accession | Q920N7 |
| Other Accession | NP_598925.1 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Description | Mouse Anti-Mouse Synaptotagmin-12 Monoclonal IgG1 |
| Target/Specificity | Detects ~45kDa. Does not cross-react with Synaptotagmin-6 or other Synaptotagmins. |
| Other Names | SYT-12 Antibody, SYT12 Antibody, Synaptotagmin 12 Antibody, Synaptotagmin XII Antibody, SytXII Antibody, Syt XII Antibody, Synaptotagmin12 Antibody, SynaptotagminXII Antibody |
| Clone Names | S277-7 |
| Immunogen | Fusion protein amino acids 168-255 (Cytoplasmic C2A domain) of mouse Synaptotagmin-12 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-437 was sufficient for detection of Synaptotagmin-12 in 20 µg of transiently overexpressing synaptotagmin-12 COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasmic Vesicle |
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Provided below are standard protocols that you may find useful for product applications.
Background
Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).
References
1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815.
2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
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