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Notch1 Antibody

Notch1 Antibody, Clone S253-32

     
  •  - Notch1 Antibody ASM10264
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (ASM10264). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (ASM10264) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Notch1 Antibody (D) Composite.
    detail
  • WB - Notch1 Antibody ASM10264
    Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (ASM10264). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (ASM10264) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q01705
Other Accession NP_032740.3
Host Mouse
Isotype IgG1
Reactivity Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Mouse Notch1 Monoclonal IgG1
Target/Specificity Detects >270kDa, ~120kDa and small fragments due to proteolysis. Does not cross-react with Notch 2 or Notch3.
Other Names Neurogenic locus notch homolog protein 1 Antibody, hN1 Antibody, Translocation associated notch protein Antibody, TAN1 Antibody, TAN-1 Antibody, Motch A Antibody, mT14 Antibody, p300 Antibody, Motch Antibody, Mis6 Antibody, Neurogenic locus notch protein homolog Antibody, NICD Antibody, Notch 1 intracellular domain Antibody, Notch gene homolog 1 (Drosophila) Antibody, Notch homolog 1 Antibody, translocation-associated (Drosophila) Antibody, NOTCH Drosophila Antibody, homolog of 1 Antibody, Translocation associated notch homolog Antibody, Translocation associated notch protein TAN 1 Antibody, xotch Antibody
Clone Names S253-32
Immunogen Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Nucleus
Research Areas
Citations (0)
citation

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Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

References

1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719.
2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.
3. Lindsell C.E., et al. (1995) Cell 80: 909-917.
4. Uyttendaele H., et al. (1996) Development 122: 2251-2259.
5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.

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Cat# ASM10264
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