Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Copper Transporting ATPase 1 Antibody
Copper Transporting ATPase 1 Antibody, Clone S60-4
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, IP |
|---|---|
| Primary Accession | Q04656 |
| Other Accession | NP_000043.3 |
| Host | Mouse |
| Isotype | IgG2b |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human Copper Transporting ATPase 1 Monoclonal IgG2b |
| Target/Specificity | Detects ~180kDa in rat brain membrane preparations. |
| Other Names | ATP7A Antibody, ATP 7A Antibody, ATPase Cu transporting Antibody, DSMAX Antibody, FLJ17790 Antibody, MC1 Antibody, MC 1 Antibody, MK Antibody, MNK Antibody, OHS Antibody, Copper pump 1 Antibody, Menke Antibody, OTTHUMP00000062077 Antibody, SMAX3 Antibody, ATPase copper transporting alpha polypeptide Antibody, ATPase Cu++ transporting alpha polypeptide (Menkes syndrome) Antibody, Copper transporting ATPase 1 Antibody, Cu++ transporting P type ATPase Antibody, Menkes disease associated protein Antibody, Menkes syndrome Antibody |
| Clone Names | S60-4 |
| Immunogen | Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-398 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody. |
| Cellular Localization | Endoplasmic Reticulum | Cytoplasm | Golgi Apparatus | Trans-Golgi Network Membrane | Cell Membrane |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.
References
1. Samimi G., et al. (2003) Clin. Cancer Res. 9: 5853-9.
2. Samimi G., et al. (2004) Mol Pharmacol. 66: 25-32.
3. Greenough M., et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.
4. Song, I.S., et al. (2004) Mol. Cancer Ther. 3: 1543-1549.
5. van Dongen, E.M., et al. (2004) Biochem. Biophys. Res. Commun. 323: 789-795.
6. Samimi, G., et al. (2004) Mol Pharmacol 66: 25-32.
7. Morgan, C.T., et al. (2004) J. Biol. Chem. 279: 36363-36371.
8. Barnes, N., et al. (2005) J. Biol. Chem. [Epub].
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
