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Ubiquitin Antibody
Ubiquitin Antibody, Clone FK2
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, IP, E |
|---|---|
| Primary Accession | P0CG47 |
| Other Accession | BAC56955.1 |
| Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human Ubiquitin Monoclonal IgG1 |
| Target/Specificity | Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin. |
| Other Names | Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody |
| Clone Names | FK2 |
| Immunogen | Ubiquitin conjugated lysozyme |
| Purification | Protein A Purified |
| Storage | -20ºC |
| Storage Buffer | 10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm | Nucleus |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
References
1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12.
2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.
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