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Ubiquitin Antibody

Ubiquitin Antibody, Clone FK2

     
  •  - Ubiquitin Antibody ASM10157
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (ASM10157). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10157) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, IP, E
Primary Accession P0CG47
Other Accession BAC56955.1
Host Mouse
Isotype IgG1
Clonality Monoclonal
Description Mouse Anti-Human Ubiquitin Monoclonal IgG1
Target/Specificity Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
Other Names Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Clone Names FK2
Immunogen Ubiquitin conjugated lysozyme
Purification Protein A Purified
Storage -20ºC
Storage Buffer 10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus
Research Areas
Citations (0)
citation

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Background

Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

References

1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12.
2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

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Cat# ASM10157
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