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Synaptophysin (native) Antibody
Synaptophysin Antibody, Clone EP10
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P08247 |
| Other Accession | NP_003170.1 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human Synaptophysin (native) Monoclonal IgG1 |
| Target/Specificity | Detects ~38kDa. |
| Other Names | SYPH Antibody, Sypl Antibody, Syn p38 Antibody, Synaptophysin Antibody, Major synaptic vesicle protein p38 Antibody |
| Clone Names | EP10 |
| Immunogen | Immunoprecipitate of human brain |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-178 was sufficient for detection of Synaptophysin in 10 µg of human brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Cell Junction | Synapse |
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Provided below are standard protocols that you may find useful for product applications.
Background
Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa (1). It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for the presynaptic vesicle, and functions in synaptic vesicle exocytosis, including cholesterol-dependent vesicle biogenesis, regulation of SNARE assembly via interaction with VAMP/synaptobrevin, formation of the fusion pore initiating neurotransmitter release, and activation of vesicle endocytosis (2, 3). This gene has also been implicated in X linked mental retardation (4).
References
1. Johnston P.A., et al. (1989) J Biol Chem. 264: 1268-1273.
2. Thiele C., et al. (2000) Nat Cell Biol. 2: 42-49.
3. Valtorta F., et al. (2004) Bioessays 26: 445-453.
4. Tarpey P.S., et al. (2009) Nat Genet. 41(5): 535-543.
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