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His-tag Antibody

     
  • WB - His-tag Antibody ASC12058
    Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 µg/ml.
    detail
  • WB - His-tag Antibody ASC12058
    Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 µg of His antibody to immunoprecipitate and 1 µg/ml anti-GGP1 antibody to detect.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IP
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Notes His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 µ/ml.
Additional Information
Other Names His-tag, His, 6xHis
PrecautionsHis-tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. His-tag is one of the more popular epitope tags for expressed recombinant proteins.

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$ 379.00
Cat# ASC12058
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