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Bim Antibody [1C2C8]
![WB - Bim Antibody [1C2C8] ASC11993](/assets/uploads/products/201512/ASC11993_WB_1.jpg)
![ICC - Bim Antibody [1C2C8] ASC11993](/assets/uploads/products/201512/ASC11993_ICC_1.jpg)
![IF - Bim Antibody [1C2C8] ASC11993](/assets/uploads/products/201512/ASC11993_IF_1.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | O43521 |
| Other Accession | NP_619527, 20336315 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 1C2C8 |
| Calculated MW | 22171 Da |
| Application Notes | Bim antibody can be used for detection of Bim by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 10018 |
|---|---|
| Target/Specificity | BCL2L11; |
| Reconstitution & Storage | Bim monoclonal antibody can be stored at -20℃, stable for one year. |
| Precautions | Bim Antibody [1C2C8] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BCL2L11 |
|---|---|
| Synonyms | BIM |
| Function | Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase- mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. |
| Cellular Location | Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. [Isoform BimL]: Mitochondrion. [Isoform Bim-alpha1]: Mitochondrion. |
| Tissue Location | Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
References
O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
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