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PUMA Antibody [10D4G7]
![WB - PUMA Antibody [10D4G7] ASC11982](/assets/uploads/products/201512/ASC11982_WB_1.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BXH1 |
| Other Accession | Q9BXH1, 56748610 |
| Reactivity | Human, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 10D4G7 |
| Calculated MW | 20532 Da |
| Application Notes | PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 µg/mL. |
| Gene ID | 27113 |
|---|---|
| Target/Specificity | BBC3; |
| Reconstitution & Storage | PUMA monoclonal antibody can be stored at -20℃, stable for one year. |
| Precautions | PUMA Antibody [10D4G7] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BBC3 |
|---|---|
| Synonyms | PUMA |
| Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). |
| Cellular Location | Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release |
| Tissue Location | Ubiquitously expressed. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References
Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.
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