PUMA Antibody [10D4G7]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, E |
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Primary Accession | Q9BXH1 |
Other Accession | Q9BXH1, 56748610 |
Reactivity | Human, Rat |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG1 |
Clone Names | 10D4G7 |
Calculated MW | 20532 Da |
Application Notes | PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 µg/mL. |
Gene ID | 27113 |
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Target/Specificity | BBC3; |
Reconstitution & Storage | PUMA monoclonal antibody can be stored at -20℃, stable for one year. |
Precautions | PUMA Antibody [10D4G7] is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BBC3 |
---|---|
Synonyms | PUMA |
Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity). |
Cellular Location | Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release |
Tissue Location | Ubiquitously expressed. |

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Provided below are standard protocols that you may find useful for product applications.
Background
PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References
Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

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