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SLC29A2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | Q14542 |
| Other Accession | NP_001523, 38708299 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 50 kDa; Observed: 50 kDa |
| Application Notes | SLC29A2 antibody can be used for detection of SLC29A2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 3177 |
|---|---|
| Target/Specificity | SLC29A2; SLC29A2 antibody is human specific. SLC29A2 antibody is predicted to not cross-react with other SLC29 proteins. |
| Reconstitution & Storage | SLC29A2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | SLC29A2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SLC29A2 (HGNC:11004) |
|---|---|
| Synonyms | DER12, ENT2, HNP36 |
| Function | Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Functions as a Na(+)-independent, passive transporter (PubMed:9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:16214850, PubMed:21795683). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed:12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed:23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity). |
| Cellular Location | Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Note=Localized to the apical membrane of Sertoli cells. |
| Tissue Location | Highly expressed in skeletal muscle (PubMed:9478986). Expressed in liver, lung, placenta, brain, heart, kidney and ovarian tissues (PubMed:9478986). Expressed in testis at the blood-brain-barrier (PubMed:23639800). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SLC29A2 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A2 is a transmembrane glycoprotein that mediates the cellular uptake of nucleosides from the surrounding medium (3). As a nucleoside transporter, SLC29A2 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4).
References
Williams JB and Lanahan AA. A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein. Biochim. Biophys. Res. Commun. 1995; 213:325-33.
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Mangravite LM, Xiao G, and Giacomini KM. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am. J. Physiol. Renal Physiol. 284:F902-10.
Owen RP, Lagpacan LL, Taylor TR, et al. Functional characterization and haplotype analysis of polymorphisms in the human equilabrative nucleoside transporter, ENT2. Drug Metab. Dispos. 2006; 34:12-5.
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