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NOX1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9Y5S8 |
| Other Accession | NP_008983, 148536873 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 62 kDa Observed: 64 kDa |
| Application Notes | NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 27035 |
|---|---|
| Target/Specificity | NOX1; NOX1 antibody is human specific. |
| Reconstitution & Storage | NOX1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | NOX1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NOX1 (HGNC:7889) |
|---|---|
| Synonyms | MOX1, NOH1 |
| Function | NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. |
| Cellular Location | Cell projection, invadopodium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein |
| Tissue Location | [Isoform NOH-1L]: Detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).
References
Helmcke I, Heumuller S, Tikkanen R, et al. Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxid. Redox Signal. 2009; 11:1279-87.
Piccoli C, D'Aprile A, Ripoli M, et al. Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species. Biochem. Biophys. Res. Commun. 2007; 353:965-72.
Lee JG, Lim EJ, Park DW, et al. A combination of Lox-1 and Nox1 regulates TLR9-mediated foam cell formation. Cell Signal. 2008; 20:2266-75.
Stanic B, Katsuyama M, and Miller FJ Jr. An oxidized extracellular oxidation-reduction state increases Nox1 expression and proliferation in vascular smooth muscle cells via epidermal growth factor receptor activation. Arterioscler. Thromb. Vasc. Biol. 2010; 30:2234-41.
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