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SNCA Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P37840 |
| Other Accession | NP_000336, 6806898 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 12 kDa Observed: 16 kDa |
| Application Notes | SNCA antibody can be used for detection of SNCA by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 6622 |
|---|---|
| Target/Specificity | SNCA; SNCA antibody is human, mouse and rat reactive. At least three isoforms of SNCA are known to exist. |
| Reconstitution & Storage | Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SNCA Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SNCA |
|---|---|
| Synonyms | NACP, PARK1 |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590). |
| Cellular Location | Cytoplasm. Membrane. Nucleus. Synapse Secreted. Cell projection, axon {ECO:0000250|UniProtKB:O55042}. Note=Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). {ECO:0000250|UniProtKB:O55042, ECO:0000269|PubMed:15282274} |
| Tissue Location | Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alpha-Synuclein (SNCA) is a hallmark of Alzheimer’s disease (1,2). It is a cytoplasmic protein that is predominantly expressed in the central nervous system (2). SNCA reduces neuronal responsiveness to various apoptotic stimuli, leading to the decreased caspase-3 activation. SNCA may be involved in the regulation of dopamine release and transport and induces fibrillization of microtubule-associated protein tau (3). Defects in SNCA are associated with familial Parkinson’s disease (4,5).
References
Ueda K, Fukushima H, Masliah E, et al. Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease. Proc. Natl. Acad. Sci. USA 1993; 90:11282-6.
Pronin AN, Morris AJ, Surguchov A, et al. Synucleins are a novel class of substrates for G protein-coupled receptor kinases. J. Biol. Chem. 2000; 275:26515-22.
Oaks AW, Frankfurt M, Finkelstein DI, et al. Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function. PLoS One 2013; 8:e60378.
Polymeropoulos MH, Lavedan C, Leroy E, et al. Mutation in the alpha-synuclein gene identified in families with Parkinson's disease. Science 1997; 276:2045-7.
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