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ME2 Antibody

     
  • WB - ME2 Antibody ASC11774
    Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2 µg/ml.
    detail
  • IHC - ME2 Antibody ASC11774
    Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 µg/mL.
    detail
  • IF - ME2 Antibody ASC11774
    Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 µg/mL.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P23368
Other Accession NP_002387, 4505145
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 64 kDa

Observed: 60 kDa
Application Notes ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Additional Information
Gene ID 4200
Target/Specificity ME2; ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
Reconstitution & Storage ME2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsME2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ME2
Function NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.
Cellular Location Mitochondrion matrix
Research Areas
Citations (0)
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Background

ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).

References

Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21.
Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
Jiang P, Du W, Mancuso A, et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013; 493:689-83.
Lenzen KP, Heils A, Lorenz S, et al. Association analysis of malic acid enzyme 2 gene polymorphisms with idiopathic generalized epilepsy. Epilepsia 2005; 46:1637-41.

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$ 379.00
Cat# ASC11774
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