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TAF9 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q16594 |
| Other Accession | NP_003178, 4507351 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 29 kDa Observed: 28 kDa |
| Application Notes | TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 6880 |
|---|---|
| Target/Specificity | TAF9; TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members. |
| Reconstitution & Storage | TAF9 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | TAF9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TAF9 |
|---|---|
| Synonyms | TAF2G, TAFII31 |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473). TAF9 is also a component of the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex (PubMed:15899866). TAF9 and its paralog TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes (PubMed:15899866). Essential for cell viability (PubMed:15899866). May have a role in gene regulation associated with apoptosis (PubMed:15899866). |
| Cellular Location | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).
References
Zawel L and Reinberg D. Advances in RNA polymerase II transcription. Curr. Opin. Cell Biol. 1992; 4:488-95.
Klemm RD, Goodrich JA, Zhou S, et al. Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation. Proc. Natl. Acad. Sci. USA 1995; 92:5788-92.
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