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Alpha-tubulin Antibody

     
  • WB - Alpha-tubulin Antibody ASC11733
    Western blot analysis of alpha-tubulin in human brain tissue lysate with alpha-tubulin antibody at (A) 0.25 and (B) 0.5 µg/ml.
    detail
  • IHC - Alpha-tubulin Antibody ASC11733
    Immunohistochemistry of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 5 µg/mL.
    detail
  • IF - Alpha-tubulin Antibody ASC11733
    Immunofluorescence of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 20 µg/mL.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q13748
Other Accession NP_005992, 17921993
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 50 kDa

Observed: 45 kDa
Application Notes Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 7278
Target/Specificity TUBA3C; Alpha-tubulin antibody is human, mouse and rat reactive.
Reconstitution & Storage Alpha-tubulin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsAlpha-tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

References

McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

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$ 379.00
Cat# ASC11733
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