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CXCL12 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | P48061 |
| Other Accession | NP_001171605, 296011023 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 15 kDa Observed: 18 kDa |
| Application Notes | CXCL12 antibody can be used for detection of CXCL12 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry starting at 5 µg/mL. |
| Gene ID | 6387 |
|---|---|
| Target/Specificity | CXCL12; CXCL12 antibody is human and mouse reactive. Multiple isoforms of CXCL12 are known to exist. |
| Reconstitution & Storage | CXCL12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | CXCL12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CXCL12 |
|---|---|
| Synonyms | SDF1, SDF1A, SDF1B |
| Function | Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner (PubMed:29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T- lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell- dependent pre-B-cells (By similarity). |
| Cellular Location | Secreted. |
| Tissue Location | Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The CXCL12 protein, also known as SDF1, is a stromal cell-derived alpha chemokine member of the intercrine family. CXCL12 functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4) and CXCR7, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis (reviewed in 1). Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections (2).
References
Timotijevic G, Mostarica Stojkovic M, and Miljkovic D. CXCL12: role in neuroinflammation. Int. J. Biochem. Cell Biol. 2012; 44:838-41.
Reiche EM, Ehara Watanabe MA, Bonametti AM, et al. The effect of stromal cell-derived factor 1 (SDF1/CXCL12) genetic polymorphism on HIV-1 disease progression. Int. J. Mol. Med. 2006; 18:785-93.
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