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TBX21 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q9UL17 |
| Other Accession | NP_037483, 7019549 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 59 kDa Observed: 55kDa |
| Application Notes | TBX21 antibody can be used for detection of TBX21 by Western blot at 1 - 2 µg/ml. |
| Gene ID | 30009 |
|---|---|
| Target/Specificity | TBX21; TBX21 antibody is human and mouse reactive. |
| Reconstitution & Storage | TBX21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | TBX21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TBX21 |
|---|---|
| Synonyms | TBET, TBLYM |
| Function | Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN- gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702). Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity). Required for the correct development of natural killer (NK) and mucosal-associated invariant T (MAIT) cells (PubMed:33296702). |
| Cellular Location | Nucleus |
| Tissue Location | T-cell specific.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box (1,2). Members of this family include transcription factors involved in the regulation of developmental processes (2). Studies in mouse and humans show that TBX21 is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (1,3). Expression of the human ortholog also correlates with interferon-gamma expression in Th1 and natural killer cells, suggesting a TBX21 may play a role in initiating Th1 lineage development from naive Th precursor cells. (3).
References
Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 2000; 100:655-69.
Naiche LA, Harrelson Z, Kelly RG, et al. T-box genes in vertebrate development. Annu. Rev. Genet. 2005; 39:219-39.
Hibbert L, Pflanz S, De Waal Malefyt R, et al. IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells. J. Interfeon Cytokine Res. 2003; 23:513-22.
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