LEMD3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9Y2U8 |
Other Accession | NP_055134, 7706607 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 99997 Da |
Application Notes | LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 5 µg/mL. |
Gene ID | 23592 |
---|---|
Target/Specificity | LEMD3; At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2 |
Reconstitution & Storage | LEMD3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | LEMD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LEMD3 |
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Synonyms | MAN1 |
Function | Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest. |
Cellular Location | Nucleus inner membrane; Multi-pass membrane protein |
Tissue Location | Heart, brain, placenta, lung, liver and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.
References
Hellemans J, Preobrazhenska O, Willaert A, et al. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. Nat. Genet. 2004; 36:1213-8.
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