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EI24 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O14681 |
| Other Accession | NP_004870, 55956766 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 38965 Da |
| Application Notes | EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. |
| Gene ID | 9538 |
|---|---|
| Target/Specificity | EI24; At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform. |
| Reconstitution & Storage | EI24 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | EI24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EI24 |
|---|---|
| Synonyms | PIG8 |
| Function | Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity). |
| Cellular Location | Nucleus membrane; Multi-pass membrane protein. Cytoplasm. Endoplasmic reticulum membrane; Multi-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
EI24 Antibody: The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.
References
Lehar SM, Nacht M, Jacks T, et al. Identification and cloning of EI24, a gene induced by p53 in etoposide-treated cells. Oncogene 1996; 12:1181-7.
Gu Z, Flemington C, Chittenden T, et al. ei24, a p53 response gene involved in growth suppression and apoptosis. Mol. Cell Biol. 2000; 20:233-41
Zhao X, Ayer RE, Davis SL, et al. Apoptosis factor EI24/PIG8 is a novel endoplasmic reticulum-localized Bcl-2-binding protein which is associated with suppression of breast cancer invasiveness. Cancer Res. 2005; 65:2125-9.
Mazumder D, Mitra S, Singh RK, et al. Inactivation of CHEK1 and EI24 are associated with the development of invasive cervical carcinoma: clinical and prognostic implications. Int. J. Cancer 2010; epub
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