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TCF12 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | Q99081 |
| Other Accession | AAH50556, 29792012 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 72965 Da |
| Application Notes | TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. |
| Gene ID | 6938 |
|---|---|
| Target/Specificity | TCF12; |
| Reconstitution & Storage | TCF12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | TCF12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TCF12 |
|---|---|
| Synonyms | BHLHB20, HEB, HTF4 |
| Function | Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity). May be involved in the functional network that regulates the development of the GnRH axis (PubMed:32620954). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TCF12 Antibody: TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
References
Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403.
Murre C, McCaw PS, Vaesin H, et al. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell1989; 58:537-44.
Wojciechowski J, Lai A, Kondo M, et al. E2A and HEB are required to block thymocyte proliferation prior to pre-TCR expression. J. Immunol.2007; 178:5717-26.
D’Cruz LM, Knell J, Fujimoto JK, et al. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells. Nat. Immunol.2010; 11:240-9.
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