APC8 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, ICC, E |
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Primary Accession | Q9UJX2 |
Other Accession | Q9UJX2, 254763423 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 68834 Da |
Application Notes | APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. |
Gene ID | 8697 |
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Target/Specificity | CDC23; |
Reconstitution & Storage | APC8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | APC8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CDC23 |
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Synonyms | ANAPC8 |
Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). |
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Provided below are standard protocols that you may find useful for product applications.
Background
APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
References
JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.
Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.
Pal M, Nagy O, Menesi D, et al. Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster. J. Cell Sci.2007; 120:3238-48.
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