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OCLN Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q16625 |
| Other Accession | AAH29886, 4950 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 57 kDa Observed: 57 kDa |
| Application Notes | OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Gene ID | 4950 |
|---|---|
| Target/Specificity | OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN. |
| Reconstitution & Storage | OCLN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | OCLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | OCLN |
|---|---|
| Function | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell junction, tight junction |
| Tissue Location | Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
OCLN Antibody: Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.
References
Tsukita S, Furuse M, and Itoh M. Multifunctional strands in tight junctions. Nat. Rev. Mol. Cell Biol. 2001; 2:285-93.
Chiba H, Osanai M, Murata M, et al. Transmembrane proteins of tight junctions. Biochim. Biophys. Acta 2008; 1778:588-600.
Furuse M, Hirase T, Itoh M, et al. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol.1993; 1777-88.
Matter K, Aijaz S, Tsapara A, et al. Mammalian tight junctions in the regulation of epithelial differentiation and proliferation. Curr. Opin. Cell Biol. 2005; 17:453-8.
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