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DDX6 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P26196 |
| Other Accession | P54824, P54823, Q5ZKB9 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54417 Da |
| Antigen Region | 348-375 aa |
| Gene ID | 1656 |
|---|---|
| Other Names | Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK |
| Target/Specificity | This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-375 amino acids from the Central region of human DDX6. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DDX6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DDX6 |
|---|---|
| Synonyms | HLR2, RCK |
| Function | Essential for the formation of P-bodies, cytosolic membrane- less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817). Plays a role in P- bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573). |
| Cellular Location | Cytoplasm, P-body. Cytoplasm. Nucleus. Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000250|UniProtKB:P54823}. Note=Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:28216671). Upon cellular stress, relocalizes to stress granules (PubMed:26184334). |
| Tissue Location | Abundantly expressed in most tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
References
Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009)
Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)
Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472(2009)
Tritschler, F., et al. Mol. Cell 33(5):661-668(2009)
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