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LXN Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LXN Antibody AP9861a
    Anti-LXN Antibody at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - LXN Antibody AP9861a
    LXN Antibody (Cat. #AP9861a) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LXN Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - LXN Antibody AP9861a
    LXN Antibody (Cat. #AP9861a) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9BS40
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25750 Da
Additional Information
Gene ID 56925
Other Names Latexin, Endogenous carboxypeptidase inhibitor, ECI, Protein MUM, Tissue carboxypeptidase inhibitor, TCI, LXN
Target/Specificity This LXN antibody is generated from rabbits immunized with human LXN recombinant protein.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLXN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LXN
Function Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. May play a role in inflammation.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in heart, prostate, ovary, kidney, pancreas, and colon, moderate or low in other tissues including brain
Research Areas
Citations (0)
citation

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Background

LXN encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases.

References

Pallares, I., et al. Proc. Natl. Acad. Sci. U.S.A. 102(11):3978-3983(2005)
Liu, Q., et al. Mol. Biol. Rep. 27(4):241-246(2000)

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$ 182.50
$ 70.00
Cat# AP9861a
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