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RT25 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P82663 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20116 Da |
| Antigen Region | 145-173 aa |
| Gene ID | 64432 |
|---|---|
| Other Names | 28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25 |
| Target/Specificity | This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RT25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MRPS25 |
|---|---|
| Synonyms | RPMS25 |
| Cellular Location | Mitochondrion. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.
References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
Hattori, A., et al. DNA Res. 7(6):357-366(2000)
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