PRUNE Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q86TP1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 50200 Da |
Antigen Region | 360-389 aa |
Gene ID | 58497 |
---|---|
Other Names | Protein prune homolog, hPrune, Drosophila-related expressed sequence 17, DRES-17, DRES17, HTcD37, PRUNE |
Target/Specificity | This PRUNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the C-terminal region of human PRUNE. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PRUNE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PRUNE1 (HGNC:13420) |
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Synonyms | PRUNE |
Function | Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis (PubMed:28334956). Involved in the regulation of microtubule polymerization (PubMed:28334956). |
Cellular Location | Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed |
Tissue Location | Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. It plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
References
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Middelhaufe, S., et al. Biochem. J. 407(2):199-205(2007)
Kobayashi, T., et al. Mol. Cell. Biol. 26(3):898-911(2006)
Zollo, M., et al. Clin. Cancer Res. 11(1):199-205(2005)
Forus, A., et al. Oncogene 20(47):6881-6890(2001)
Reymond, A., et al. Oncogene 18(51):7244-7252(1999)
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