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NPW Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8N729 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 18048 Da |
| Antigen Region | 121-150 aa |
| Gene ID | 283869 |
|---|---|
| Other Names | Neuropeptide W, Preproprotein L8, hPPL8, Neuropeptide W-23, NPW23, hL8, Neuropeptide W-30, NPW30, hL8C, NPW, PPL8, PPNPW |
| Target/Specificity | This NPW antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the C-terminal region of human NPW. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NPW Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NPW |
|---|---|
| Synonyms | PPL8, PPNPW |
| Function | Plays a regulatory role in the organization of neuroendocrine signals accessing the anterior pituitary gland. Stimulates water drinking and food intake. May play a role in the hypothalamic response to stress (By similarity). NPW23 activates GPR7 and GPR8 more efficiently than NPW30. |
| Cellular Location | Secreted |
| Tissue Location | Detected in cerebrospinal fluid and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717) Detected at high levels in the substantia nigra, fetal kidney and trachea; at lower levels in testis, uterus, ovary and placenta. Not detectable in many regions of the central nervous system. Also detected at high levels in lymphoblastic leukemia and colorectal adenocarcinoma |
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Provided below are standard protocols that you may find useful for product applications.
Background
NPW is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade. The preproprotein is translated using a non-AUG initiation codon that is inferred from analyses of the mouse ortholog. [provided by RefSeq].
References
Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Singh, G., et al. Br. J. Pharmacol. 148(8):1033-1041(2006)
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