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WIPI2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WIPI2 Antibody (C-term) AP9559b
    Western blot analysis of WIPI2 Antibody (C-term) (Cat. #AP9559b) in mouse brain tissue lysates (35ug/lane). WIPI2 (arrow) was detected using the purified Pab.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y4P8
Other Accession Q6AY57, Q80W47, Q5ZHN3
Reactivity Human, Mouse
Predicted Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49408 Da
Antigen Region 426-454 aa
Additional Information
Gene ID 26100
Other Names WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2, WIPI2
Target/Specificity This WIPI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human WIPI2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWIPI2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WIPI2 (HGNC:32225)
Function Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:20505359, PubMed:28561066). Involved in an early step of the formation of preautophagosomal structures (PubMed:20505359, PubMed:28561066). Binds and is activated by phosphatidylinositol 3- phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed:28561066). Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P (PubMed:28890335). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:20505359, PubMed:28561066).
Cellular Location Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.
Tissue Location Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down- regulated in pancreatic and in kidney tumors
Research Areas
Citations ( 0 )

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).

References

Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

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$ 182.50
$ 70.00
Cat# AP9559b
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