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YIPF5 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - YIPF5 Antibody (N-term) AP9554b
    Western blot analysis of YIPF5 Antibody (N-term) (Cat. #AP9554b) in MDA-MB231 cell line lysates (35ug/lane). YIPF5 (arrow) was detected using the purified Pab;
    detail
  • WB - YIPF5 Antibody (N-term) AP9554b
    Western blot analysis of YIPF5 Antibody (N-term) (Cat. #AP9554b) in CHO cell line lysates (35ug/lane). YIPF5 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - YIPF5 Antibody (N-term) AP9554b
    YIPF5 Antibody (N-term) (Cat. #AP9554b) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YIPF5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q969M3
Other Accession Q4R5M4
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27989 Da
Antigen Region 21-49 aa
Additional Information
Gene ID 81555
Other Names Protein YIPF5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, Smooth muscle cell-associated protein 5, SMAP-5, YIP1 family member 5, YPT-interacting protein 1 A, YIPF5, FINGER5, YIP1A
Target/Specificity This YIPF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the N-terminal region of human YIPF5.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYIPF5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YIPF5 (HGNC:24877)
Synonyms FINGER5, YIP1A
Function Plays a role in transport between endoplasmic reticulum and Golgi. In pancreatic beta cells, required to transport proinsulin from endoplasmic reticulum into the Golgi (PubMed:33164986).
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9EQQ2}; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle {ECO:0000250|UniProtKB:Q5XID0}. Note=Enriched at the endoplasmic reticulum exit sites (By similarity). Incorporated into COPII-coated vesicles (By similarity). {ECO:0000250|UniProtKB:Q5XID0, ECO:0000250|UniProtKB:Q9EQQ2}
Tissue Location Ubiquitously expressed with abundant expression in pancreatic tissue, islets, beta cells, and brain. Highly expressed in coronary smooth muscles.
Research Areas
Citations (0)
citation

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Background

Plays a role in transport between endoplasmic reticulum and Golgi.

References

?Koskinen, L.L., et al. Tissue Antigens 74(5):408-416(2009)
?Kano, F., et al. J. Cell. Sci. 122 (PT 13), 2218-2227 (2009) :
?Yoshida, Y., et al. Exp. Cell Res. 314(19):3427-3443(2008)
?Jin, C., et al. Biochem. Biophys. Res. Commun. 334(1):16-22(2005)
?Stolle, K., et al. Gene 351, 119-130 (2005) :

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$ 365.00
$ 140.00
Cat# AP9554b
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