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PCNA Recombinant Rabbit mAb, Nuclear Loading Control

PCNA Recombinant Rabbit mAb, Nuclear Loading Control

     
  •  - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Blank control:Hela. Primary Antibody (green line): Rabbit Anti-PCNA antibody (AP94695) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PCNA Recombinant Rabbit mAb, Nuclear Loading Control AP94695
    Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA (Nuclear Loading Control) Monoclonal Antibody, Unconjugated(AP94695) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Reactivity Human
Host Rabbit
Clonality Recombinant
Additional Information
Dilution WB=1:1000-10000,IHC-P=1:200-1000,IHC-F=1:200-1000,ICC/IF=1:100-500,IF=1:200-1000,Flow-Cyt=1ug/Test
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
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citation

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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94695
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