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PI3-kinase p110 subunit beta Recombinant Rabbit mAb

PI3-kinase p110 subunit beta Recombinant Rabbit mAb

     
  • WB - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human K562 cell lysates Lane 3: Human Jurkat cell lysates Primary: Anti-PI3-kinase p110 subunit beta (AP94694) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kD Observed band size: 110 kD
    detail
  •  - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PI3-kinase p110 subunit beta) monoclonal Antibody, unconjugated (AP94694) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
    detail
  • IHC-P - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(AP94694) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(AP94694) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(AP94694) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(AP94694) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
  • IHC-P - PI3-kinase p110 subunit beta Recombinant Rabbit mAb AP94694
    Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3-kinase p110 subunit beta Monoclonal Antibody, Unconjugated(AP94694) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q24JU2
Reactivity Human
Host Rabbit
Clonality Recombinant
Additional Information
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:200,IF=1:100-500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Research Areas
Citations (0)
citation

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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94694
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