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SLC1A5 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q15758 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 56598 Da |
| Antigen Region | 186-214 aa |
| Gene ID | 6510 |
|---|---|
| Other Names | Neutral amino acid transporter B(0), ATB(0), Baboon M7 virus receptor, RD114/simian type D retrovirus receptor, Sodium-dependent neutral amino acid transporter type 2, Solute carrier family 1 member 5, SLC1A5, ASCT2, M7V1, RDR, RDRC |
| Target/Specificity | This SLC1A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human SLC1A5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SLC1A5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SLC1A5 {ECO:0000303|PubMed:23756778} |
|---|---|
| Function | Sodium-coupled antiporter of neutral amino acids. In a tri- substrate transport cycle, exchanges neutral amino acids between the extracellular and intracellular compartments, coupled to the inward cotransport of at least one sodium ion (PubMed:17094966, PubMed:23756778, PubMed:26492990, PubMed:29872227, PubMed:34741534, PubMed:8702519). The preferred substrate is the essential amino acid L- glutamine, a precursor for biosynthesis of proteins, nucleotides and amine sugars as well as an alternative fuel for mitochondrial oxidative phosphorylation. Exchanges L-glutamine with other neutral amino acids such as L-serine, L-threonine and L-asparagine in a bidirectional way. Provides L-glutamine to proliferating stem and activated cells driving the metabolic switch toward cell differentiation (PubMed:23756778, PubMed:24953180). The transport cycle is usually pH-independent, with the exception of L-glutamate. Transports extracellular L-glutamate coupled to the cotransport of one proton and one sodium ion in exchange for intracellular L-glutamine counter-ion. May provide for L-glutamate uptake in glial cells regulating glutamine/glutamate cycle in the nervous system (PubMed:32733894). Can transport D-amino acids. Mediates D-serine release from the retinal glia potentially affecting NMDA receptor function in retinal neurons (PubMed:17094966). Displays sodium- and amino acid-dependent but uncoupled channel-like anion conductance with a preference SCN(-) >> NO3(-) > I(-) > Cl(-) (By similarity). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Tissue Location | Placenta, lung, skeletal muscle, kidney, pancreas, and intestine (PubMed:8702519). Expressed in CD34-positive hematopoietic progenitors (at protein level) (PubMed:24953180) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SLC1A5 (Solute carrier family 1 (neutral amino acid transporter), member 5) is a member of the Na(+)-dependent amino acid transporter superfamily. It has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. It acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses.
References
# Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
# Wich, C., et al. Gynecol. Obstet. Invest. 68(1):9-18(2009)
# Avissar, N.E., et al. Dig. Dis. Sci. 53(8):2113-2125(2008)
# Broer, S. Physiol. Rev. 88(1):249-286(2008)
# Deng, X., et al. BMC Psychiatry 8, 58 (2008)
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