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phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb

phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb

     
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (AP94133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (AP94133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (AP94133) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3 Kinase p110 beta(Ser1070)) Polyclonal Antibody, Unconjugated (AP94133) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    detail
  • WB - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human K562 cell lysates Primary: Anti-phospho-PI3 Kinase p110 beta (Ser1070) (AP94133) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110-123 kD Observed band size: 110 kD
    detail
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3 Kinase p110 beta (Ser1070) ) Polyclonal Antibody, Unconjugated (AP94133) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - phospho-PI3 Kinase p110 beta (Ser1070) Rabbit pAb AP94133
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-PI3 Kinase p110 beta (Ser1070) ) Polyclonal Antibody, Unconjugated (AP94133) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Primary Accession Q24JU2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50,IF=1:100-500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Research Areas
Citations (0)
citation

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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94133
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