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PI3 Kinase p110 beta Rabbit pAb

PI3 Kinase p110 beta Rabbit pAb

     
  • WB - PI3 Kinase p110 beta Rabbit pAb AP94117
    Sample: Jurkat(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti- PI3 Kinase p110 beta (AP94117) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kD Observed band size: 123 kD
    detail
  •  - PI3 Kinase p110 beta Rabbit pAb AP94117
    Blank control:A549. Primary Antibody (green line): Rabbit Anti-PI3 Kinase p110 beta antibody (AP94117) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
  • WB - PI3 Kinase p110 beta Rabbit pAb AP94117
    Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti- PI3 Kinase p110 beta (AP94117) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 123 kD Observed band size: 123 kD
    detail
  • IHC-P - PI3 Kinase p110 beta Rabbit pAb AP94117
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta) Polyclonal Antibody, Unconjugated (AP94117) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - PI3 Kinase p110 beta Rabbit pAb AP94117
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI3 Kinase p110 beta) Polyclonal Antibody, Unconjugated (AP94117) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E
Primary Accession Q24JU2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Additional Information
Dilution WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=3ug/test,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Research Areas
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94117
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