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TUBA3E (Tubulin alpha 3e) (16K16) Mouse Monoclonal antibody
TUBA3E (Tubulin alpha 3e) (16K16) Mouse Monoclonal antibody
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TUBA3E antibody (AP93915), and then analyzed by flow cytometry.
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Anti-TUBA3E mouse monoclonal antibody (AP93915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA3E .
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA3E. Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | Q6PEY2 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Calculated MW | 49859 Da |
| Gene ID | 112714 |
|---|---|
| Other Names | Tubulin alpha-3E chain, 3.6.5.-, Alpha-tubulin 3E, Detyrosinated tubulin alpha-3E chain, TUBA3E |
| Storage Conditions | -20℃ |
| Name | TUBA3E |
|---|---|
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Cellular Location | Cytoplasm, cytoskeleton. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).
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