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KBTBD4 (5G8) Mouse Monoclonal antibody

KBTBD4 (5G8) Mouse Monoclonal antibody

     
  •  - KBTBD4 (5G8) Mouse Monoclonal antibody AP93883
    Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KBTBD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93883) (1:500)
    detail
  •  - KBTBD4 (5G8) Mouse Monoclonal antibody AP93883
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KBTBD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KBTBD4 antibody (Cat# AP93883). Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9NVX7
Reactivity Human, Mouse
Clonality Monoclonal
Calculated MW 59904 Da
Additional Information
Gene ID 55709
Other Names Kelch repeat and BTB domain-containing protein 4, BTB and kelch domain-containing protein 4, KBTBD4, BKLHD4
Storage Conditions-20℃
Protein Information
Name KBTBD4
Synonyms BKLHD4
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex which targets CoREST corepressor complex components RCOR1, KDM1A/LSD1 and HDAC2 for proteasomal degradation (PubMed:33417871). RCOR1 is likely to be the primary target while degradation of KDM1A and HDAC2 is likely due to their association with RCOR1 (PubMed:33417871). Also targets RCOR3, MIER2 and MIER3 for proteasomal degradation as well as associated proteins ZNF217 and RREB1 (PubMed:36997086). Degradation is dependent on the presence of an ELM2 domain in the target proteins (PubMed:36997086).
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Cat# AP93883
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