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BTLA (6U2) Mouse Monoclonal Antibody

BTLA (6U2) Mouse Monoclonal Antibody

     
  •  - BTLA (6U2) Mouse Monoclonal Antibody AP93636
    25 ng of recombinant human BTLA was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. AP93636 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. BTLA band was visualized using ECL Substrate. Result: AP93636 can detect human BTLA by Western blotting.
    detail
  •  - BTLA (6U2) Mouse Monoclonal Antibody AP93636
    IHC-P analysis of human tonsil tissue by anti-human BTLA antibody (AP93636). IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with anti-human BTLA primary antibody (AP93636) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Non-germinal center cells and a few of germinal center cells are positively stained at the cell membrane.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q7Z6A9-2
Reactivity Human
Clonality Monoclonal
Additional Information
Storage Conditions-20℃
Protein Information
Citations (0)
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$ 385.00
Cat# AP93636
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