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Anti-FABP6 Antibody

Rabbit polyclonal antibody to FABP6

     
  • WB - Anti-FABP6 Antibody AP93283
    Western blot analysis of FABP6 expression in MCF7 (A), A375 (B) whole cell lysates.
    detail
  • IF - Anti-FABP6 Antibody AP93283
    Immunofluorescent analysis of FABP6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF/IC
Primary Accession P51161
Other Accession P51162
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 14371 Da
Additional Information
Gene ID 2172
Other Names ILBP; ILLBP; Gastrotropin; GT; Fatty acid-binding protein 6; Ileal lipid-binding protein; ILBP; Intestinal 15 kDa protein; I-15P; Intestinal bile acid-binding protein; I-BABP
Target/Specificity Recognizes endogenous levels of FABP6 protein.
Dilution WB~~WB (1/500 - 1/1000)
IF~~IF/IC (1/50 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name FABP6
Synonyms ILBP, ILLBP
Function Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes (By similarity). In vitro binds to bile acids in the order: deoxycholic acid > cholic acid > chenodeoxycholic acid and respective BA conjugation modifies affinities in the order taurine-conjugated > glycine-conjugated > unconjugated bile acids. Stimulates gastric acid and pepsinogen secretion (By similarity).
Cellular Location [Isoform 1]: Cytoplasm {ECO:0000250|UniProtKB:P80020}. Membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P50119}; Cytoplasmic side {ECO:0000250|UniProtKB:P50119}
Tissue Location Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa (at protein level).
Research Areas
Citations (0)
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Background

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human FABP6. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP93283
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