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GPX1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IHC-P, FC, E |
|---|---|
| Primary Accession | P07203 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 22088 Da |
| Antigen Region | 164-193 aa |
| Gene ID | 2876 |
|---|---|
| Other Names | Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1 |
| Target/Specificity | This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1. |
| Dilution | IF~~1:10~50 WB~~1:2000 IHC-P~~1:10~50 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPX1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPX1 (HGNC:4553) |
|---|---|
| Function | Catalyzes the reduction of hydroperoxides in a glutathione- dependent manner thus regulating cellular redox homeostasis (PubMed:11115402, PubMed:36608588). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:11115402, PubMed:36608588). In platelets catalyzes the reduction of 12-hydroperoxyeicosatetraenoic acid, the primary product of the arachidonate 12-lipoxygenase pathway (PubMed:11115402). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P11352}. Mitochondrion {ECO:0000250|UniProtKB:P11352} |
| Tissue Location | Expressed in platelets (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
Background
GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.
References
Moyer,A.M., et.al., Cancer Epidemiol. Biomarkers Prev. 19 (3), 811-821 (2010)
Akimoto,A.K., et.al., Free Radic. Res. 44 (3), 322-331 (2010)
Cao,C., et.al., J. Biol. Chem. 278 (41), 39609-39614 (2003)
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