DENN Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | Q8WXG6 |
Reactivity | Rat |
Clonality | Monoclonal |
Other Names | IG20; MADD; RAB3GEP; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 183303 Da |
Dilution | WB 1:500~1:2000 IHC 1:50~1:200 |
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Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from human DENN |
Description | Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | MADD {ECO:0000312|EMBL:AAB57735.1, ECO:0000312|HGNC:HGNC:6766} |
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Function | Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed:18559336, PubMed:20937701). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed:18559336, PubMed:20937701). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A- positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed:32761064). May link TNFRSF1A with MAP kinase activation (PubMed:9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed:11577081, PubMed:32761064). |
Cellular Location | Cell membrane. Cytoplasm. Cell projection, axon {ECO:0000250|UniProtKB:Q80U28} |
Tissue Location | Expressed in testis, ovary, brain and heart (PubMed:8988362). Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon (PubMed:9115275). Expressed in liver (PubMed:9796103). [Isoform 2]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid [Isoform 4]: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid [Isoform 6]: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid |
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