HIF Prolyl Hydroxylases Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, IP |
---|---|
Primary Accession | Q9NXG6 |
Clonality | Monoclonal |
Other Names | EGLN4 ; HIFPH4; Hypoxia inducible factor prolyl 4 hydroxylase; P4H with transmembrane domain; P4htm; PH4; PHD4; Proline 4 hydroxylase; Prolyl hydroxlase domain containing 4; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 56661 Da |
Dilution | WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50 |
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Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from human HIF Prolyl Hydroxylases |
Description | Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | P4HTM |
---|---|
Synonyms | PH4 |
Function | Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
Tissue Location | Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. |
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