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ATP1B2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ATP1B2 Antibody (Center) AP9271c
    Western blot analysis of lysates from K562 cell line and rat liver tissue lysate(from left to right), using ATP1B2 Antibody (Center)(Cat. #AP9271c). AP9271c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
    detail
  • IHC-P - ATP1B2 Antibody (Center) AP9271c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATP1B2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - ATP1B2 Antibody (Center) AP9271c
    ATP1B2 Antibody (Center) (Cat. #AP9271c) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P14415
Other Accession P13638, Q8WMG3, P14231, Q28030
Reactivity Human
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33367 Da
Antigen Region 115-141 aa
Additional Information
Gene ID 482
Other Names Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule in glia, AMOG, Sodium/potassium-dependent ATPase subunit beta-2, ATP1B2
Target/Specificity This ATP1B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP1B2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP1B2
Function This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Cellular Location Cell membrane; Single-pass type II membrane protein
Research Areas
Citations (0)
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Background

The protein belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

References

Guey,L.T., et.al., Eur. Urol. 57 (2), 283-292 (2010)
Tokhtaeva,E., et.al., Biochemistry 48 (48), 11421-11431 (2009)
Hosgood,H.D. et.al., Respir Med 103 (12), 1866-1870 (2009)

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$ 365.00
$ 140.00
Cat# AP9271c
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