EFEMP2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
---|---|
Primary Accession | O95967 |
Other Accession | Q9WVJ9, O55058 |
Reactivity | Human, Mouse |
Predicted | Hamster |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 49405 Da |
Antigen Region | 386-414 aa |
Gene ID | 30008 |
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Other Names | EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, FIBL-4, Protein UPH1, EFEMP2, FBLN4 |
Target/Specificity | This EFEMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human EFEMP2. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EFEMP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EFEMP2 (HGNC:3219) |
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Synonyms | FBLN4 |
Function | Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed:27339457). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (By similarity). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed:18973305, PubMed:19570982). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed:23782690). |
Cellular Location | Secreted, extracellular space, extracellular matrix Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:Q9WVJ9}. Note=Localizes on the microfibrils surrounding ELN cores. {ECO:0000250|UniProtKB:Q9WVJ9} |
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Provided below are standard protocols that you may find useful for product applications.
Background
EFEMP2 have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein contains four EGF2 domains and six calcium-binding EGF2 domains. This protein is necessary for elastic fiber formation and connective tissue development.
References
Chen,Q., et.al., Biochem. J. 423 (1), 79-89 (2009)
Choudhury,R., et.al., J. Biol. Chem. 284 (36), 24553-24567 (2009)
El-Hallous,E., et.al., J. Biol. Chem. 282 (12), 8935-8946 (2007)
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