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ERGIC3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ERGIC3 Antibody (N-term) AP9217a
    Western blot analysis of ERGIC3 Antibody (N-term) (Cat. #RB22586) in HepG2 cell line lysates (35ug/lane). ERGIC3 (arrow) was detected using the purified Pab.
    detail
  • WB - ERGIC3 Antibody (N-term) AP9217a
    Western blot analysis of ERGIC3 (arrow) using rabbit polyclonal ERGIC3 Antibody (N-term) (Cat. #AP9217a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ERGIC3 gene.
    detail
  • IHC-P - ERGIC3 Antibody (N-term) AP9217a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ERGIC3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y282
Other Accession Q9CQE7, Q4R8X1, Q803I2, Q5EAE0
Reactivity Human
Predicted Bovine, Zebrafish, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43222 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 51614
Other Names Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84, ERGIC3, C20orf47, ERV46, SDBCAG84
Target/Specificity This ERGIC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ERGIC3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERGIC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERGIC3
Synonyms C20orf47, ERV46, SDBCAG84
Function Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615).
Cellular Location Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi-pass membrane protein. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Cycles between the endoplasmic reticulum and the Golgi
Research Areas
Citations (0)
citation

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Background

ERGIC3 is possible role in transport between endoplasmic reticulum and Golgi.

References

Nishikawa,M., et.al., Gene 386 (1-2), 42-51 (2007)
Breuza,L., et.al., J. Biol. Chem. 279 (45), 47242-47253 (2004)

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$ 182.50
$ 70.00
Cat# AP9217a
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