EB3 Antibody
Rabbit mAb
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, ICC |
---|---|
Primary Accession | Q9UPY8 |
Reactivity | Rat |
Clonality | Monoclonal |
Other Names | EB3; EBF3; MAPRE3; RP/EB Family; RP3; |
Isotype | Rabbit IgG |
Host | Rabbit |
Calculated MW | 31982 Da |
Dilution | WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50 |
---|---|
Purification | Affinity-chromatography |
Immunogen | A synthesized peptide derived from human EB3 |
Description | May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. |
Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Name | MAPRE3 |
---|---|
Function | Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:19255245, PubMed:28814570). Promotes microtubule growth (PubMed:19255245, PubMed:28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed:19255245, PubMed:28814570). Also acts as a regulator of minus- end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). |
Cellular Location | Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Detected at the plus end of microtubules |
Tissue Location | Predominantly expressed in brain and muscle. |
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