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POU2AF1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POU2AF1 Antibody (N-term) AP9096a
    Anti-POU2AF1 Antibody (N-term) at 1:2000 dilution + Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - POU2AF1 Antibody (N-term) AP9096a
    Anti-POU2AF1 Antibody (N-term) at 1:2000 dilution + Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - POU2AF1 Antibody (N-term) AP9096a
    POU2AF1 Antibody (N-term) (Cat. #AP9096a)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - POU2AF1 Antibody (N-term) AP9096a
    POU2AF1 Antibody (N-term) (Cat. #AP9096a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession Q16633
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27436 Da
Antigen Region 1-27 aa
Additional Information
Gene ID 5450
Other Names POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1
Target/Specificity This POU2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human POU2AF1.
Dilution WB~~1:2000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU2AF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU2AF1 (HGNC:9211)
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
Cellular Location Nucleus.
Tissue Location B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290)
Research Areas
Citations (0)
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Background

POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

References

Pittman,A.M., et.al., Hum. Mol. Genet. 17 (23), 3720-3727 (2008)
Gashaw,I., et.al., Mol. Hum. Reprod. 13 (10), 721-727 (2007)

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$ 365.00
$ 140.00
Cat# AP9096a
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