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PPM1H Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPM1H Antibody (Center) AP9093c
    Western blot analysis of PPM1H Antibody (Center) (Cat. #AP9093c) in K562, 293, MCF-7, Jurkat cell line and mouse spleen tissue lysates (35ug/lane). PPM1H (arrow) was detected using the purified Pab.
    detail
  • IHC-P - PPM1H Antibody (Center) AP9093c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PPM1H Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - PPM1H Antibody (Center) AP9093c
    PPM1H Antibody (Center)(Cat. #AP9093c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q9ULR3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56448 Da
Antigen Region 235-263 aa
Additional Information
Gene ID 57460
Other Names Protein phosphatase 1H, PPM1H, ARHCL1, KIAA1157, URCC2
Target/Specificity This PPM1H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human PPM1H.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPM1H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPM1H
Synonyms ARHCL1, KIAA1157, URCC2
Function Dephosphorylates CDKN1B at 'Thr-187', thus removing a signal for proteasomal degradation.
Cellular Location Nucleus. Cytoplasm
Citations ( 0 )

References

Sugiura,T., et.al., Cancer Biol. Ther. 7 (2), 285-292 (2008)
Labes,M., et.al., Mol. Cell. Neurosci. 12 (1-2), 29-47 (1998)

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$ 365.00
$ 140.00
Cat# AP9093c
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