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CDK4 Antibody
Rabbit mAb
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- CITATIONS
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- BACKGROUND
Application 
| WB, ICC, IP |
|---|---|
| Primary Accession | P11802 |
| Reactivity | Rat |
| Clonality | Monoclonal |
| Other Names | Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; PSKJ3; CDK4; Crk3; CMM 3; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 33730 Da |
| Dilution | WB 1:1000~1:2000 ICC/IF 1:50~1:200 IP 1:50 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human CDK4 |
| Description | Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Name | CDK4 |
|---|---|
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Cellular Location | Cytoplasm. Nucleus. Nucleus membrane. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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$ 350.00
$ 175.00
Cat# AP90836
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